Ayaya or AYAYA (for Twitch Emotes) is a memorable yet somewhat annoying quote from Karen Kujo of Kin-iro Mosaic. In the anime adaptation of scene, Karen misidentifies the character "Aya" (aka. Aya Komichi) by calling her "Ayaya." Initially the scene was parodied in various remixes (eg. YouTube videos, GIF reaction etc.) and became internet meme some point around mid-2010.

Few years later, a user called FoveVever submitted the Karen face in the scene to FrankerFacez as Twitch emotes and then the rest is history. It spread like horde of bees in Twitch chat and became prominent as rival of Gachi.
This emotes usually describe when someone or something refer to anything Anime, Manga and Japanese stuff in Twitch.

Examples 1:
Meanwhile in Admiralbulldog Twitch chat section,
Plebs: AYAYA Clap AYAYA Clap AYAYA Clap AYAYA Clap AYAYA Clap AYAYA Clap
Admiralbulldog: Your banned for 3 Days, sir. *Proceed banning the plebs*
Other Twitch chat user: BANNED OMEGALUL
Gachi user: gachiBASS WEEBS AWAY gachiBASS
Twitch Subs: Weebs SoBayed

Examples 2:
Someone donate streamer for playing Japanese related song or videos,
Twitch User: AYAYA Clap AYAYA Clap AYAYA Clap AYAYA Clap AYAYA Clap AYAYA Clap
Streamer: Sorry i am stop playing it, is annoying and pretty cringe. *Proceed stop the song/videos*
Twitch User: BAKA!! PunOko

Examples 3:
The gif below:

AYAYA by IAmNotGoodInEnglish😂👌 March 27, 2019