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Ruh Roh was originally one of the many iconic Scooby-Doo sayings but has been turned into a phrase used at times of wrongdoing or mistake to make people laugh or lighten the mood.
Ruh Roh we're behind on our rent!

Ruh Roh I farted in the hand dryer

Ruh Roh The Po-Po
Ruh Roh by bored.exe August 29, 2022
A Hanna-Barbera canine cartoon saying for "Uh-oh," beginning with THE JETSONS' Astro (1962) and continuing with Scooby-Doo in the Seventies.

Astro tended to speak a kind of English except that words with beginning consonants were replaced with an "R," and "R" was inserted in front of vowels.

Scooby-Doo was less fluent, but was given "Ruh-roh" as a kind of running gag when things were going bad.

Now "Ruh-roh" is sometimes used as a jocular trope where dogs are concerned, as in a recent news story about a dog who was accidentally released by Air Canada from his cage at the San Francisco airport. Rough meaning: "I goofed" or "I'm in trouble."
"Astro, if you don't stop that you're going to be in big trouble."

"Ruh-roh."

"Alright Astro, you asked for it, no outdoor privileges all weekend."

"Raw, Rorge!"
Ruh-roh by al-in-chgo October 11, 2013
Guy 1: OH SHIT MY HOUSE BURNED DOWN!
Guy 2: RUH ROH!ahhahaha
ruh roh by remright September 6, 2003
Scooby Doo's comedic mispronunciation of "Uh Oh", it is also a play on potentially dire situations, usually starting with a minor event that could escalate into something huge.
North Korea just shelled a South Korean island? Ruh Roh!

There is a small leak from the dam? Ruh Roh!
ruh roh by sanjuro_kurosawa November 26, 2010

Ruh Roh Raggy 

A common phrase used by Scooby Doo or many others, often to show fear or intense emotions.
Ruh Roh Raggy Fred is jacking off to among us again!
expression used in place of "uh oh"
derived from the popular cartoon show, Scooby Doo
Kelly: "That girl hooked up with Shaniqua's boyfriend, she's gonna get the shit kicked out of her during lunch today."

Amanda: "RUH-ROH."
ruh-roh by chiquitaLOCAA January 3, 2009