"are you serious?" in a positive way
do not mistake with "no me jodas" which means the same in a negative way or "don't fuck with me", "leave me alone"
(This exact definition is only applied for Spanish from Spain but it is also used maybe in other context in some other Hispanic countries)
"No joda" is a spanish slang (commonly used by Puerto Ricans, Dominicans, etc.) Depending on your tone, saying "no joda" can either mean "Don't mess with me" or if you say it with an attitude it can mean "don't fuck with me".