HPLC Share definition Flag A common analytical lab technique which stands for High-performance liquid chromatography; known to most chemists as high pain level for chemist. The HPLC of aspirin gave like 5 different peaks, what the hell is all this. HPLC by mschneider1 May 28, 2007 0 0 Get merch Get the HPLC mug. Buy Now — $32.95 See more merch