As per Know Your Meme, the expressionoriginated from 4chan in late 2010, and is used by trolls to "convey feelings of discontent or discomfort in response to someone else’s post in imageboards and discussion forums".
The expression is an alternate form of "u mad bro?".
as others have stated: something has angered, upset, annoyed, aroused you
this is more of a side note on origin - I suspect the phrase was a spontaneous misquote of a line from the Weezer song "Say It Ain't So" ("...flip on the telly/ wrestle with Jimmy...")
The act of fucking up someone's shit to the point they can no longer continue to live with them selves because of how bad someone just ruined their day
“That Really Rustled My Jimmies” is an expression that is used to convey feelings of discontent or discomfort in response to someone else’s post in imageboards and discussion forums, most notably on 4chan. To rustle one's jimmies means to make someone discontent or to bother someone.