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Rustle My Jimmies 

“That Really Rustled My Jimmies” is an expression that is used to convey feelings of discontent or discomfort in response to someone else’s post in imageboards and discussion forums, most notably on 4chan. To rustle one's jimmies means to make someone discontent or to bother someone.
Man 1: "This guy is your boyfriend? He's worthless!"

Man 2: "That really does Rustle My Jimmies man..."
Rustle My Jimmies by Carlos-a-16 December 12, 2012

rustle your jimmies 

Stevens the type of guy to rustle your jimmies
rustle your jimmies by Clazza 1999 November 30, 2016

Rustle My Jimmies 

Repositioning sexual organs. For males, repositioning of the penis and testicles. For females, repositioning of the breasts.
In awkward situations I like to rustle my jimmies
Rustle My Jimmies by Skewesy October 20, 2013

rustled jimmies 

To disturb someone by presenting them with facts/arguments that cause cognitive dissonance and challenge their views.
You know why people start arguments on the internet? Rustled jimmies man.

Are your jimmies rustled? 

jimmies – feelings / thoughts / ego
rustled – hurt / disturbed
unrustled – peaceful / calm

As per Know Your Meme, the expression originated from 4chan in late 2010, and is used by trolls to "convey feelings of discontent or discomfort in response to someone else’s post in imageboards and discussion forums".

The expression is an alternate form of "u mad bro?".
Victim: What the heck is your problem!?
Troll: Are your jimmies rustled?
Victim: ...What is this I don't even
Troll: *trollface*

Whatever Rustles your Jimmies

Person 1: Hey man, what do you want to eat?
Person 2: Oh, I don't care. Whatever rustles your Jimmies.