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"Pootis" is a quote from the Heavy, from Valve's free-to-play Team Fortress 2 game. The original quote by said Heavy is "Put dispenser here". However, due to his accent, it appears to sound like he is saying "Pootis spencer here". Therefore, people have grabbed the word "Pootis" from this, thus creating an internet meme.

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Pootis Team Fortress 2 Team Fortress Heavy Heavy Weapons Guy
Pootis by Outspace June 24, 2013
Heavy: POOTIS
Pootis by Mattdiction October 14, 2015
Meaning 1 : "Pootis" or "Pootus", ~noun, Phu-tos the meaning of life.
Meaning 2 : A Phrase removed from TF2's Put Dispenser Here. Used by the Heavy Weapons Guy of TF2
Example 1 :Scout: Yo Heavy
Heavy: What?
Scout: Define "Pootis"
Heavy: "Pootis" or "Pootus", ~noun, Phu-tos the meaning of life.
Example 2: Heavy : Put Dis/penser here.
Pootis by SirKnowsALotDictionary September 16, 2015

pootis spencer 

The phonetic spelling of the way the "Heavy" character from Team Fortress 2 requests a dispenser, (an in-game item built by the Engineer character that gives health and ammo to nearby players), saying "Put dispenser here" though his accent makes it sound rather difficult
Pootis or noun~ phu-tos
It is the meaning of life.
*cry everytime*
I love pootis. Its the meaning of life.
Pootis by I AM NOT DEADPOOL July 31, 2017

Pootis Pow 

A special skill of advanced TF2 hoovies where they first of all equip either the Stock, Saxxy, Apoco-fists or Holiday punch, taunt, and quickly say the "put dispenser here" voice-line. This gives the effect of, if done right, saying "Pootis Pow"
Hoovy: *pretends hand is gun* pootis POW haha!
Pootis Pow by Bottt_urban April 14, 2021