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1: Alternative and often but not always offensive, way of spelling "Jew", in the religious or racial sense. It originated from South Park.

2: Way of spelling "You". It is used sometimes in 1337 (leet). It probably originated from some European languages, such as Dutch, having "J" be pronounced "Y".
1: "Maybe we're not getting to heaven because somebody here is a J-O-O" - South Park.

"Jon Stewart is a filthy joo" - antisemite.
"I didn't know he was jooish" - person.

2: "Joo w1ll g3t teh fl@g!!!" - gamer playing "capture the flag"
"Joo @r3 ST00P1D!!!1!!!one!!!" - gamer expressing his/her (who am i kidding, HIS) frustration with a fellow gamer whom he perceives as being incompetent or unintelligent.

"Ja" means "yes" in Dutch, and is pronounced "Ya".
Related Words
joo joob joog joons jooks Joop Joost joonas joo'd Jooonah
Internet slang for the word "you." Most often used by 1337 computer freaks and/or white, male, black wanna-be's.
H3y, J00! Y0 m4mm4 w45 600d 1457 ni6h7, F00!
joo by SizzlinGyrl August 7, 2003
Also used as "you", commonly mispronounced by Cubans.
"What joo talkin' 'bout, mang?"
Joo by Lucifer May 8, 2004
It is another way of saying you. Used mostly by hardcore gamers.
I fucking pwn joo son.
Joo by Fuckphace August 29, 2006
joo by CFX February 2, 2003