Variation of, "you are a liar" or "quit clowning". Indeginous to the American South, specfically Mississippi.
As Jimbo ends his "Yo Mama" joke ( directed towards Leroy), Leroy counters with, "You a lie."
by millhouse May 8, 2004
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Something some students in Washington say when a friend, family member, etc tells a lie.

Roots: "You a Lie" is used most frequently, and was brought up from the TV show "Meet the Browns."
Josh: So what did you do in Arizona?

Jacob: I invented the teleportation device.

Josh: YOU A LIE!
by JPdude6 April 10, 2010
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The classiest way to respond to anyone you disagree with.
Obama: There are also those who claim that our reform efforts would insure illegal immigrants. This too is false - the reforms I'm proposing would not apply to those who are here illegally.

Joe Wilson: You lie!
by illicit_fun_owns September 10, 2009
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What Michelangelo shouted at Shredder when Shredder claims that Splinter is dead.
"You lie!"

"Do I?"
by jorgorthebarbarian September 10, 2009
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What Leonardo shouted at Shredder when Shredder claimed that Splinter was dead.
Raphael: Where's Splinter?

Shredder: Ah, the rat. So it has a name. It had a name.

Leonardo: You lie!

Example 2:

jorgorthebarbarian: "You lie!" is what Michaelangelo shouted at Shredder when he claimed that Splinter was dead.

Me: You lie!
by shouldn't have apologized wuss September 28, 2009
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The (obviously) most classy way of politely telling someone that they are wrong. Take it from Joe.
Obama: I like pie.

Joe: YOU LIE!
by jazjaz130 September 11, 2009
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The easiest way to object to a statement for which you have no legitimate argument.
Obama: There are also those who claim that our reform efforts would insure illegal immigrants. This too is false - the reforms I'm proposing would not apply to those who are here illegally.

Joe Wilson: You lie!
by stating the obvious September 13, 2009
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