The sensation that a spider is crawling on you when it is not. This usually comes directly after finding a spider on you and can last for days after.
Sam: Betty, is there a spider on me?!
Betty: No, dude, it's just your spidey senses.
Sam: Yeah, I've been this way ever since I found a spider on me when I was in the bath the other day.
Derived from the "Spidey sense" of the comic book superhero Spiderman, it is generally used to mean a vague but strong sense of something being wrong, dangerous, suspicious, a security situation.
He looked like a student, but something about his dirty shoes and the way he tried to open a locked door totally set off my Spidey sense, so I called campus security.
1) I told that woman I felt a instant connection to her and that I knew we were soul-mates. Then I asked for her number but the gal's spidey sense must have been tingling because she just stood right up without saying anything and got off the bus.
2) That lady's spidey sense must have been working overtime that day because as soon as I started talking to her she gave me one of those "I'm about to start screaming" faces and then moved to another seat at the far end of the subway car.
the cyber spidey sense, when someone has the ability to supersense someone's emotions, even through the cold interface of a chatbox. usually referred to HCSS or magical powers.