Nee-sama 

Means sister in Japanese often people who watch the popular Anime Re:Zero will use it.

Rem: Nee-sama Nee-sama, could our guest be a troubled man, deriving pleasure from torture?
Ram: Rem, Rem our guest appears to be a pervert who's turned on by our insults.

Nee-sama by Vesy November 16, 2017

Nee-sama 

Means "sister" in Japanese, mostly used by rem from Re:zero

Rem: "nee-sama ,nee-sama"
Ram: "rem ,rem"

Nee-sama by Satania Is kawaii March 19, 2022