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Absolutely the most annoying phrase Texans say! I have lived down here the past 14 years and have never gotten used to it. It still rubs my Yankee ears the wrong way.

It means "I can't hear you or I don't understand".
Person Number 1: "Well, I blah, blah, blah..."

Person Number 2: "Do what?" (usually said in a rude tone)
do what by Ex-Yankee September 21, 2006
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the most annoying phrase that our neighbors use
"hey come and play airsoft"
"DO WHAT?"
do what by sausage and eggs April 5, 2008
A ghetto term mainly used to exclaim a misunderstanding with what was said. See also what.
Ghetto Guy: ::Mumble, mumble, junk::
Ghetto Chick: Do what?
do what by Roger Sullivan July 3, 2006
When you’re about to beat the hell out of someone for a rude/disrespectful comment or question but you like the person so you are giving them the chance to make it right.
Man: Hey, you wanna fuck?
Woman: Do what?

Man: Is that a no?
Woman: Beats that ass and not in a good way.
Do What by Want2FlyAwayWithMe April 25, 2020

Do what? 

Huh, what, what'd you say?
Do what? by BFC Brookfield Crew January 6, 2008

DO WHAT? 

A slang term used by southerners when they don't under stand what you just told them: (eg): "Someone should be calling you with an appointment within two business days." Billy Bob: "Do what, noww?"
eg): "Someone should be calling you with an appointment within two business days." Billy Bob: "Do what, noww?"

How are ya doing today?

"Do what?"
DO WHAT? by yeahyeahyeahmarv November 13, 2005

do what? 

1. a redneck response when one doesn't hear/understand what someone just said.

2. a reaction when surprised at someone's statement.
california boy: i'm totally stoked
redneck: do whaaat?

hot girl in bar- i was born a man
boy who just made out with hot girl- do what?
do what? by jsllskdf October 11, 2007