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It means act like a man, but in a non-sexist way. It is not meant to be chauvinistic or disparage other groups. Similar phrases would be "be a man," "be a mensch," "be a stand-up guy" or "wake up and accept responsibility for your actions."
Judge Marilyn Milian on THE PEOPLE'S COURT occasionally uses the term "man up" in the way described above, somewhat like an earlier TV instruction of hers to "butch up your act" but not so gay-identified. She has been provoked to the "man up" remark when confronted with such clueless men as the biological father who claimed he 'wanted to be a daddy' when he in fact had not visited his son nor contributed to his support, and the man who consistently maintained he had put only a scratch on plaintiff's car when in fact all the witnesses confirmed and all the photographic evidence showed that he had done considerable damage. It would be absurd for Judge Milian to order a woman to "man up" but she has other, analogous remarks, to make to similarly witless or hypocritical women.
Man Up by al-in-chgo February 20, 2010
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Man Period 

An area of wetness discovered on the seat of your chair due to condensation from the concentrated, excess heat dissipating from your ass.
I stood up to leave, when I looked down at my chair and realized I was on my man period. Now the girls next to me think my ass is leaking.
Man Period by Mr. Zhgé November 10, 2009
Related Words
man man whore mangina mana manny Mango mang mansplaining manu Mandy

Man Child 

A man child is a early twenties ex-frat boy who looks like a big fat fucking baby in grown man clothing. When seen in their natural habit (a bar infested with man children) they're usually talking about how lucrative their lifestyles are while in reality they're bank tellers, depositing my checks.
Man Child: Yeah bro, I'm working in Insurance; I should clear half a mil a year soon.

Andrew: You aren't doing shit. You are going to be one of those kids I hire to do all my running around, taking care of all my bullshit.
Man Child by Skarbar January 11, 2011
Step one: Check if your balls are still attached.
Step two: They are? Good.
Step three: Check again. Odds are you're drunk.
Step four: Still there? Awesome. Then just Do It.
"I dunno, that guy's pretty huge, I think he might even have a hundred pounds on me. Are you sure I can take him?"
"Of course, man. Now, what the hell are you waiting for? Man up, and take that fat bastard Bubba down!"
Man Up by Dropkick December 28, 2005
A Man J is derived from the French word Mange. A Man J is the man version of the San J; when a man shits into another mans asshole.
Zach was trashed out of his mind and gave his friend Matt a mad Man J.
Man J by Brosweetz August 3, 2009
When a guy wears his sunglasses on his head as a douchey fashion statement.
Dude, it's cloudy. Time to retire the man tiara.
Man Tiara by dizzle815 July 11, 2010
A man who is a perfect 10. Dead sexy. A Greek God
Person 1: Did you hear that David Beckham and Brad Pitt are having a baby?

Person 2: Yeah. Definitely would fuck that baby. what a Man Dime
Man Dime by reeferdachiefer May 17, 2011