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- To grow some balls, as it pertains to the pursuit of women. The act of "suiting up" is a realization of values that make a man attractive to a woman, and a rejection of the values of a man that are idealized in women, but provide no success pragmatically in attraction. This includes rejecting notions of being nice, seeking to be liked for whom you are, and treating a woman well etc. Hence, "suiting up" is a moment of growth and acceptance of such things necessary to attraction as playing hard to get, being rich, being cocky, having good hygiene, and dressing well. This phrase is derived from the physical action of putting on a suit or "suiting up" in order to impress the ladies.
Barney: Hey, did you get lucky on your date last night?

Ted: I treated her with the utmost respect and attention and she told me never to call her again.

Barney: Suit up, buddy.

Joe: Im so nice to women and all they do is shit on me. Time to suit up!
suit up by Steven Brinks November 18, 2010
Suit up simply means 'grow a pair and let's go'. Throwing a suit on doesn't hurt either.
Barney: Ted we're going out tonight.

Ted: Nah, I'm going to stay in and be a lame ass.

Barney: Ted, we're going out tonight. Suit up!

Ted: But...

Barney: Suit up!!!

Ted: Okay
Suit up by Ayblinkin December 7, 2014
A term popularized by a Twitch Streamer known by the name of xKeynote. Which in terms means to get on the game or stop what you are doing to play something.
Omar : Yo, i'm bored Gangy, I don't know what to do.
Key : Suit Up, We need to get level 420 Today.
Suit Up by dwfromarthur February 18, 2022
To apply, in the case of a male, a condom, before sexual intercourse.
"Laurie, I've gotta suit up before you can ride."
Suit up by rayrayrayrayrayrayray November 23, 2009
The call of the native "overdresser". Men who know no bounds of "overdressing" understand this in the context of the phrase.
"We are going to party, Suit up!"

"We are going to party, Birthday Suit up!"

"We are going to party, Flight Suit up!"

etc., etc., etc.
Suit Up by The Renegade Monkey December 17, 2008
1) A desperate attempt to dress like Neil Patrick Harris.

2) To "be awesome".
Suit Up by Porgo July 6, 2011