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"It's Ma'am" is a quote taken from a viral video in which a trans woman proclaims "It's ma'am" while accusing an employee of misgendering at a GameStop retail store in Albuquerque, New Mexico. After which IT had a dick fit and start to throw things onto the ground.
Look at that ma'am looking tranny . only a blind person would believe that shit
Word addressed when referring to an older women, that should never be used to address a women younger than 40.
“Hello ma’am do you need help crossing the street?”
Ma’am by Thankyouverymuchb August 8, 2019
Person: What’s good ma’am how are you
Guy said to: I’m doing nice bro
Ma’am by Carleyisabot October 2, 2019
Overly used term in the South of phony respect, just

Iike all other forms of “respect” in the South. Used to downplay a woman’s accomplishments. Synonym of b**ch.
Woman: Could you get those reports to me ASAP?
Eye rolling worker: yes ma’am
Ma’am by StormiC September 17, 2018
an individual full of logic and/or leaving you SHOOK AF
Ma’am you’re being petty. It’s ICONIC!!!
Ma’am by allyghostkatt March 13, 2019

Ma’am, this is a Wendy’s 

A response to someone giving a long-winded, serious rant that is typically unasked for. Likely to have originated either from The Office, or is a variation on “Sir, this is an Arby’s”.
Anna: “-and honestly I think that’s why my relationship with my father is so strained, he was there but never really present. Sometimes I wish things were different, but’s there’s no changing the past, you know?”
Cashier: “Ma’am, this is a Wendy’s”