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This is speaking of canid animals (members of the dog family; Canidae). A knot is basically a swelling of the Bulbus Glandis, a part of a canine's penis. The erectile tissue swells up during the climax of sexual intercourse and locks when it penetrates the female allowing it to inseminate into the female canine

this is referred as tying the knot
"the dog tied the knot in his doggy girlfriend now they are stuck there till the ball shrinks"
(i'm so dirty)
Knot by LexxDef June 26, 2019
Related Words
A person who you refuse to admit to a relationship ship with due to shame, embarrassment, or already being in a relationship. Or a loose or sexually active person you might complicate your life with.
“My girl is trippin over a “Knot” from the other night. Might have to cut her off, can’t have loose ends and get caught up. Can’t have another public “entanglement”.
Knot by TheLost0311 July 11, 2020
A knot is a wad of cash, a clump of money, a roll of bills, cash money.
A girl says " if you want to go with me then drop another knot".
Knot by Turbo uber X February 4, 2022
An esoteric multiplex often heard as a "Not" by the wicked, but understood at great depth by the wise, usually used in complex preaching situations with other words that also have double meanings
Joseph Smith threw a KNOT in there! Romans 4:5 JST
Knot by StillTrying2Help May 19, 2023
Plural for feet entwined
“Have you seen lots of feet?”
Oh yes! Under the cover I saw hattie and Dan’s feet entwined, that’s called a knot”
Knot by Cwtcher March 31, 2024

knot-so-fresh 

That fucking filthy after-shit feeling that just will not go away without baby wipes.
Dude, you okay? You were in there a long time.
Fuck no. An entire roll of tp can't get rid of this knot-so-fresh feeling.
knot-so-fresh by sciflyer.25 September 4, 2014