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A relatively new interrogative phrase, independent of the preceding verb tenses, used to emphasize the extreme nature of the preceding clause.

The phrase has its origins in the Atlanta Public School System. In its most common incarnation, it's said with a humorous, yet sassy look over one's glasses, or general "girlfrand!" attitude.
FUCK HIM!

(You mad, ain't it?!?!)

or

(You ain't mad, is it!?!)
by Nicholas de Lenfent April 7, 2009
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A termed of agreement used most commonly in the Gary-Chicago area.Can be used to replace "hell yeah" and other positive affirmations. Short for "ain't it the truth"
This weed is the shit!
(ain't it!)
by gravity5 March 5, 2006
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A phrase used to express to the other person that his/her struggles are not as serious as the speakers', that they would never understand the gravity of your hardship.
From Trippie Redd's "This Ain't That":

"Nigga's be talkin' shit but they ain't with that shit for real
Fuck nigga talkin' shit then he gon' get his ass killed
Fuck nigga talkin' shit then he gon' get his ass spilled
You ain't really real, you ain't with that shit for real
Nigga this ain't that for real, this ain't that for real
You's a bitch, you's a lame nigga, this ain't that for real
You's a bitch, you should quit, nigga, this ain't that for real
This ain't that for real, this ain't that for real
No legit, you's a bitch, nigga, this ain't that for real
Odell with the pick, nigga, this ain't that for real
I'm a fucking walking lick, but nigga this ain't that for real"
by primalmeme August 14, 2020
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slang contraction of is not, are not, am not, do not or does not.
He ain't lazy, he just ain't got nothing to do.
by superrockstar125 February 11, 2003
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informal language;teachers hate it;used in texting,talking
Ain't nobody got time for that.
She ain't listening to you
Why ain't anybody texting me today
by Iisreallystupid101 November 1, 2015
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The response said when someone says something that just isn't morally right, or disturbing.
Brad: "I just nutted on my cousins face!"
Tyquan: "Yeah, no that ain't it."
by I HERPED WHILE I DERPED! July 10, 2018
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That ain't it is just a more edgy or trendy way of saying No or disagreeing to something.
"That ain't it", Simon says as Caleb talked about eating cake with lasagne
by RoyaltySyd January 28, 2019
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