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Gno (or gnoe, plural: gnoes) is a bastardized version of "No," applied in a manner to make it seem silly. The original objective was to parody people who were starting threads on message boards with topics like "OH NO!!!!!!1111"

It was first employed afterwards in limited amounts on chat sites, by people mocking other people that cannot spell - or at least those who choose not to.

Only recently has it really started to resurface on the web, and in particular, IRC. Jaimas, a regular IRC Chatter has repeatedly used it on his channel, and several others have taken a liking to the saying.
"Oh teh gnoes!"

"Oh gno!"
Gno by Jaimas August 24, 2004
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G- Get
N- Nob
O- Out

Thus literally meaning get your nob out
Usually used as an insult or as an obvious sex phrase
Boy: You're a loner and you've got no friends (Insulting)
Boy2:GNO

Or

Boy: Wanna have sex?
Girl: GNO!
GNO by The Pro One March 11, 2009
Related Words
gnome gnoming Gnomed Gno gnocchi gnodab gnomie gnoc gnostic Gnob
Bro ghoulnut and nigroid (rip 4k) are in GNO and make fire OC
GNO by SpicyBoi2Dank4U September 30, 2018

Gno/Gino 

have you seen that hot guy Gno/Gino He is so pretty
When Saying "I Know." Just isn't enough.
Marissa: "There's Only 30 Days of school left!"

Holly: "I GNO I GNO"
I Gno by HollyxBoxBolly May 10, 2010

gnomesnag 

If someone were to take something of yours in an extremely aggressive manner.
Person A: "Can you believe that I was just gnomesnagged in broad daylight!?"
Person B: "Like the girls that have been snagged from me."

Person A: "Whenever I play Dota 2, Rubick always steals my ult and we lose our teamfight gnomesang?"
Person B: "Man Rubick is such a gnomesnag"

Person A: "I am soo hungry that I could gnomesnag a sandwich at the local sandwich shop!"
Person B: "Don't be rude!"
gnomesnag by DTMango February 10, 2014

gnomesang 

An internet gamer slang for "You Know What I'm Saying?"
"Your Pentium 4 laptop cannot run Dota 2 gnomesang?"

"You n00bs are gna lose badly gnomesang!"

"You got a test tomorrow, maybe you should study gnomesang?"
gnomesang by DTMango January 20, 2013