Nee-sama 

Means sister in Japanese often people who watch the popular Anime Re:Zero will use it.

Rem: Nee-sama Nee-sama, could our guest be a troubled man, deriving pleasure from torture?
Ram: Rem, Rem our guest appears to be a pervert who's turned on by our insults.

Nee-sama by Vesy November 16, 2017

nee-san 

Japanese noun for "sister".
-san being a touch of respect.
-chan for cute

nee-chan is cute @_@

nee-san by NiZ December 15, 2003

Nee-san 

Casual Japanese noun meaning Older Sister. Onee-san is the formal form of this noun.

Onee and Onii (Older Brother) do not have to have the ending -san, -kun, -chan, or -sama
See Nii-San; Onii-san; Ototo-kun; Imoto-chan

Nee-san, when are we going to the mall?

姉、私たちがいつモール街に行きます

Onee-san, watashitachi gaitsu mo^ru machi ni iki masu?

Nee-san by Khana Hatake January 1, 2010

Nee-sama 

Means "sister" in Japanese, mostly used by rem from Re:zero

Rem: "nee-sama ,nee-sama"
Ram: "rem ,rem"

Nee-sama by Satania Is kawaii March 19, 2022

Nee-San 

Nee-San (Pronunciation-Nè Sa)

Meaning-Big Sister

Also:-Nee Chan is generally regarded formal for Elder sister.

Arigatou,Nee-San.
(Thank you, Sister)

Nee-San by AlXN January 28, 2017

Bitchy née san 

you’re cringey and a dumb anime fan. So if you’re a dumb anime fan, I will call you Bitchy née San. If you’re a smart anime fan, Ill call you a cool anime fan.

You’re such a bitchy née San.

Bitchy née san by Pure vanilla cookie! August 19, 2022

Nee Naw Siren Blasting Bitches 

Polices.

"Let's go, the nee naw siren blasting bitches are here!"

Nee Naw Siren Blasting Bitches by Adarthus October 19, 2021