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Wearing the number 2 headband 

Reference to Afro-Samurai, the popular Anime starring Samuel L. Jackson (voice) as the title character.

In a futuristic yet feudal Japan, it is said that the one who wields the Number 1 headband is the fiercest fighter in the world and shall possess godlike powers. The only way to obtain the Number 1 headband is to challenge and defeat him in combat. However, only the Number 2 can challenge the Number 1, while anyone can challenge the Number 2, which causes a constant struggle for the Number 2 headband.

This is used when it seems like EVERYONE has a bone to pick with you in a given day
dude, my girlfriends pissed at me, my roommate's on my case, my co-workers left me with a pile of work and my boss says im on thin ice -- I must be wearing the number 2 headband

Door Number 2 

Basically, the same thing as the poop chute, butthole, anus, or whatever flavor-of-the-week saying you have where you excrete feces.
Dude, that chick definitely wanted me to put it in door number 2.
Door Number 2 by J. Saynt April 15, 2009

rule number 2 

i'm gonna walk away, you know rule number 2?
rule number 2 by phatskinnyguy August 3, 2019

Arby's number 2 

When a women has partaken in sexual activity too often and is left with pussy lips that can be compared to an Arby's roast beef sandwich
Man 1 "dude I think I'm going to try and sleep with Jessica."
Man 2 " nah man I heard she's got an Arby's number 2."
Man 1 "forget it that's disgusting."
Arby's number 2 by Tito Hughes December 11, 2014

step number 2 

In South Park the TV show, Stan, Kyle, Kenny and Cartman found a place underground where gnomes work constantly; step number one is to steal underwear, step three is to take over the world and no one knows what step two is.
Stan:"So what do you do?"
Gnome:"Step one, steal underpants. Step three take over the world!!"
Stan:"So what is step number 2?"
Gnome 1 :*pauses*"um, hey guys what's step two?"
Gnome 2 :*shruggs* "I don't know."

Rule Number 2 

Keith is a dick
Mr. Keith was being bitched at about a students grade and responded with, rule number 2
Rule Number 2 by theceo November 30, 2011