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A sexual practice whereby a group of male friends (or "homies") queue up and take turns fucking girls. This adds a systematic element to group sex, much like the Miller rotation system does in volleyball.
From 2 Live Crew's "We Want Some Pussy":

"You see, me and my homies like to play this game
We call it Amtrak but some call it the train
We all would line up in a single-file line
And take our turns at waxing girls' behinds
But every time it came to me, I was shit out of luck
Because I'd stick my dick in, and it would get stuck
The girls would say "Stop!" I'd say "I'm not!
That's enough, I quit, 'cause y'all are bustin' me out!"
I say, girls, don't hide it, just divide it
And please don't knock it until you've tried it
So to all of you bitches and all you hoes
Let's have group sex and do the Rambo!"
Amtrak by sc0ner June 14, 2010
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The act of passing a slut between one of more guys who each takes a turn putting it in one or more of her holes.

See train
That girl rode the amtrak all night until she ran out of oil.
Amtrak by Jeff Killeen June 17, 2003
from amtrak old moto We always pull out on time.... the act of pulling out berfore u come inside a girl's pussy
I was fucking this girl but i had to amtrak it because i wasnt wearing a condom.
Amtrak by lax guy 4114 December 9, 2007
verb. To have sex without a condom.

derived from: America's national passenger rail system. It's a lot of fun to ride, but it's really dangerous. It always seems like a train is derailing or crashing.
Girlfriend: I'm ready, put it in!

Guy: But I don't have protection, maybe we shouldn't...

Girlfriend: It's OK, just AMTRAK me!
amtrak by amtracker April 25, 2010
america's way to travel with weed
amy poehler loves me...she said so herself. jealous much!?
amtrak by marni March 22, 2005
when a person is out of control, a complete train wreck
He was an amtrak last night
amtrak by Sally free-bird August 22, 2007
A Communist type system of government pseudo-corporate monopoly on the US passenger rails lines. If you want to book a commerical train passage your only choice is AmTrak and a market of small time tour bussinesses.
My homey took the AmTrak to Houston a few weeks ago.
AmTrak by mirkane July 3, 2004