When someone states that one is a gender other than one identifies. Misgendering is most common when it comes to transgender individuals, but can happen to cisgender people as well. (Example: Being called "Sir" when "Ma'am" is appropriate, etc...)

When you eat dirt for the first time and leave your family to build a shack in the woods

When a person is assumed to be another gender than their gender identify and/ or expression, through someone's actions,thoughts,etc.