It means act like a man, but in a non-sexist way. It is not meant to be chauvinistic or disparage other groups. Similar phrases would be "be a man," "be a mensch," "be a stand-up guy" or "wake up and accept responsibility for your actions."
Judge Marilyn Milian on THE PEOPLE'S COURT occasionally uses the term "man up" in the way described above, somewhat like an earlier TV instruction of hers to "butch up your act" but not so gay-identified. She has been provoked to the "man up" remark when confronted with such clueless men as the biological father who claimed he 'wanted to be a daddy' when he in fact had not visited his son nor contributed to his support, and the man who consistently maintained he had put only a scratch on plaintiff's car when in fact all the witnesses confirmed and all the photographic evidence showed that he had done considerable damage. It would be absurd for Judge Milian to order a woman to "man up" but she has other, analogous remarks, to make to similarly witless or hypocritical women.
by al-in-chgo February 20, 2010
Get the Man Up mug.
Step one: Check if your balls are still attached.
Step two: They are? Good.
Step three: Check again. Odds are you're drunk.
Step four: Still there? Awesome. Then just Do It.
"I dunno, that guy's pretty huge, I think he might even have a hundred pounds on me. Are you sure I can take him?"
"Of course, man. Now, what the hell are you waiting for? Man up, and take that fat bastard Bubba down!"
by Dropkick November 19, 2005
Get the Man Up mug.
Sticky Fingaz's song that was featured on the video game Def Jam: Fight For New York
That song is Hot New Shit.
by wcgold December 26, 2005
Get the Man Up mug.
The process of performing a set of eccentric body motions in order to fend off ill omen
Ty: Oh man Carl she broke that mirror man that's bad luck

Carl: That's bad luck man

Ty: Man we gotta man up dawg

(man up has occured)

Carl: Broke dat Hex
by Moobycow June 28, 2009
Get the Man Up mug.
Become more brave
What? You need to man up. Do some push ups and man up, dog.
by iwill December 18, 2002
Get the Man Up mug.
Said when the would be, baller is talking all the shit to get what he/she wants
Another way of being called out after action has been taken.
You want it sucka, it's time to Man up. Request or ask for what you want and see what happens.

or

Guy: Bro I put $20 on the fact J's bullshittin.

Girl: Yeah he won't do it! Says he's a bad ass baller but won't just go for it. Man up dude.
Guy: Nevermind $20. I put a 50 spot on the fact he pussies out and doesn't do it.
by wolfdog8mynutsDrnsqirl November 26, 2009
Get the Man Up mug.
to build up enough courage (usually for men) to face adversity and responsibility
When someone man-up its shows maturity and increases respect from society.
by Gerard Irick April 16, 2010
Get the man-up mug.