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1.
An organized or impromptu gathering of people that use Twitter. (A meet up of people that 'tweet' using Twitter.)
Are you going to the "tweetup" tonight?
by @stephenpc November 09, 2008
 
2.
A gathering of nerds attempting social contact, likely for the first time. Usually disintegrates into everyone running to the nearest computer to type to one another.
Tommy: Hey Jethro, quit playing WoW its time to meet some folks at the tweetup!

Jethro: Oh yeah I forgot about that, I can't wait to meet BigBootay421 at the tweetup, but first I need to slay this orc!
by SteveBonus August 05, 2009
 
3.
Taking social media one step further and meeting the people from twitter, as a group, that you socialize with on a daily basis.
him: Did you hear about that tweetup in Canada?

her: You mean #tweetONeh? I was there! It was the best tweetup ever!
by tweetup attendee June 24, 2013
 
4.
Basically a meet-up, but for cunts.
ass1:
@ass2 hey, u going to the DC tweet-up?

ass2:
@ass1 yeah boyyee!
by Rohaq March 12, 2009
 
5.
A group of friends on Twitter (social network) that are planning to meet up. A request by a user to meet with friends via Twitter.
Hey @user1 and @user2, u guys wanna tweetup at the Nickelback concert at Mariner?
by Blaede January 19, 2009