Aperture Science's greatest creation, the Genetic Lifeform and Disk Operating System; or GLaDOS, is a super computer with an 'infinite capacity for knowledge' who runs the Aperture Science Computer-Aided Enrichment Center.

GLaDOS' personality is made up by four 'cores'- spheres loaded with data that instill a sense of curiosity, morality, logic (or, alternatively, cake or memory), and anger into her system. The Morality core is notorious for failing, or being blocked, allowing her to go on rampages that end in the death of Aperture Science employees (by way of deadly neurotoxic gas), and run insane tests for the good of all of us.
GLaDOS will never value your safety over your unique ideas and creativity.
by Dr. Paine August 17, 2009
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Some random AI that has two different appearances in Portal 1 and 2.
Hella tryna poison me with neurotoxin, failed two times.
Muthafuka sent Wheatley and Space Core a free trip to space (no returns).
Has once been fit into a potato. Nicknamed PotatOS.
Bro: Hey wanna eat some mashed potato for dinner?
Me: Hell naw. It might be GLaDOS infected. I don’t wanna poison someone with neurotoxin.
by The Gaiaphage April 8, 2019
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GLaDOS is the massive supercomputer of Apeture Science. GLaDOS is one of the only 2 main characters in the first Portal, and is still a main character in Portal 2. GLaDOS had different cores plugged into the sides of her. The last core was plugged in late, but this was because she flooded the Enrichment Centre with a deadly nerotoxin, the core was the Morality core (Followed on from her curisoty, anger and inteligence cores) and was supposed to stop her from flooding the enrichment centre. When Chell meets GLaDOS, GLaDOS mentions a suprise, but instead of the suprise (most likely going to be nerotoxin) her morality core falls out. Trying to tell Chell to leave it alone, Chell incinerates it, and she starts flooding the enrichment centre again. GLaDOS stands for Genetic Lifeform and Disk Operating System. GLaDOS also was killed by Chell, but re-awakened by Wheatly and Chell a later few thousand years on, and want to continue testing.
GLaDOS's Morality core causes her to lie.

Example:

GLaDOS: You are doing, very well!
GLaDOS: You will be baked, and then, there will be cake.
by Hunter4271 July 4, 2011
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A Super Computer/Robot from the PC/Xbox Game Portal. Known to have a passive aggressive personality disorder, and an obsession with cake.
by timmy2022 December 11, 2007
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GLaDOS (Genetic Lifeform and Disk Operating System,1 DOS being a homage to Microsoft's MS-DOS) is a fictional artificially superintelligent computer system from the video game series Portal. GLaDOS later appeared in The Lab and Lego Dimensions. The character was created by Erik Wolpaw and Kim Swift and voiced by Ellen McLain. GLaDOS is responsible for testing and maintenance in the Aperture Science Computer-Aided Enrichment Center in all titles.
Lol if only glados was real...
by badass cajun resistor II December 9, 2020
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a term to describe something or someone who is gay

a gay person in astonia 3.5
who is that in c5? oh its glados
by lulzpwnt August 24, 2008
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A dumbass half-assed impression of GLaDOS made by a Turkish retard
A GLaDOS Domi with a robot voice : wow you are useless
Guy : Bruh
by DayVed December 24, 2020
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