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Something you say after you said something really gay and you're trying to hide the fact that you're gay as fuck
Owo I wanna suckle on that throbbing cock, no homo
No Homo by Fuckin'Normie March 5, 2018
A phrase said after something that may be misconstrued as homosexual.
I'm gonna fuck you in your ass and cum inside you, then you gonna eat the cum that drip out, whiteboy. No homo.
no homo by RapeUboyz July 7, 2006
It is added in a sentence:
1. to make it sound less gay.
2. to say if you're not kidding; Seriously.
1. Paul and I had each other's back all the time, no homo.
2. No homo though, Paul was a good friend.
no homo by joooon August 24, 2005
An interjection used to counteract a statement or act that might be interpreted as expressing homosexual desires.

As of late, the word has humorously evolved to counter a statement that has even the slightest tinge of homosexuality to it.
Some of my favorites from the internet:

"...commanding the dirty funked up wah-wah guitar, lush arrangements, heartstopping strings, wailing double-tracked backing vocals and trippy backwards fx to do backflips and weave their way in and out of macked-out horns and throbbing bass grooves (no homo!) while crisp snares and reverberating bongos underline the whole experience."

And even funnier (to me):

"A quick search on froogle (no homo) pulls up 160 Gigs of external harddrive (again, no homo) for as cheap as $106."
no homo by inktostaintreeskin October 6, 2006
No homo prevents all gayness or bisexual allegations or sayings for example if you were to say something gay or said you’d do something that is gay or sounds gay, etc. saying the words no Homo nullifies the gayness of the certain sentence or claim
No Homo by NasTheGoat April 25, 2019
A phrase used to either deny or imply a sexual innuendo, usually out of homophobia or simply to make something you or someone else says sound clever or humorous.
"if he doesn't like it he can kiss my ass, no homo"
or
person 1: "A bunch of sea men were eaten by whales in a tragic accident today."
person 2: "no homo."
no homo by Nacho January 2, 2005