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TL;DR for both cases - Easily killing an enemy.

In MMORPG's: To attack a lone member of the enemy team with many people via gang rape; To be a high level character and 1v1 someone that's a very low level and has no chance of winning or even significantly harming the aggressor.

In MOBA's: To secure an easy kill by way of abilities that allow the aggressor to close in on an enemy or cause a high amount of damage in a short amount of time, or both, whilst the victim is out of place. AKA to come up and have suprise anal with that retarded tank who's overextended and isolated on midlane.
Elitist Noob Tank: This fucking Khaimera keeps ganking me, gankers are basically just playing on easy mode, tanks actually take skill.
Ganker: Lmao don't step out of line then fucknut, you're an easy pick.
Ganking by TheRealTronaldDump June 14, 2017
To kill,steal,beat,grab and yank.
1. The words grab and yank mushed together to make ganking.
2.To repeatedly kill a virtual game character after they get resurrected aka spawn rape in WoW.
3. Group of thugs or gangsters beat and steal your money.
Ganking by kuh-rissie December 22, 2008
Oh my God, they are ganking us! It's an ambush !
ganking by Quetzacotl December 24, 2007
A sexual position where a man will coat his testicles in peanut butter, and submerge them into a woman's mouth, who will have her teeth and tongue coated in cream cheese.
Bro, me and Taylor had a insane Ganking session last night!
Ganking by BigC_ April 30, 2023
An imaginary means of killing someone 'unfairly' in WoW.

Ganking, by definition of the game is quite impossible, because killing people of the enemy faction in any way, even if looked down upon is the object of the game.

If ganking wasn't the intention of the developers, it would be taken out. Plain and simple.
-'Can you help me? This level 60 is ganking me!'

-'No, because that's what he's supposed to do'

-'I don't understand!'
ganking by Laminator May 13, 2005
Incorrectly used word in warcraft (Dota, WoW). Noobs mistakingly think infinite form of gang=ganking. Some even use the word "gank" itself even though the word gank means either to steal or to hurt one's feelings according to dictionary.com
N00b_Sux0r: "these guys are gay, they keep ganking me. "
Me: wtf is "ganking"? you mean "ganging"?
N00b_Sux0r: yeah, whatever. Doesn't matter, someone set us up the bomb.
Ganking by Thiago Santos January 10, 2006