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Originally a non-slang word, ain't began as a contraction for "am not", as in "I ain't stupid." When people began to use it improperly as in "He ain't", which should have been "He isn't", people came to consider it improper use of language. Today, it's known to be used predominantly in the south and by uneducated people.
I ain't yo' momma.

He ain't goin' to eat those nasty baked beans.
ain't by Pitoyable May 2, 2005
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You ain't Smuckers

Used to imply in a satirical manner that someone is "Jelly" (jealous).

It is often used in question form, see example.
Guy 1: Dude, your girlfriend looks like crap today.
Guy 2: Breh, you ain't Smuckers. So why are you so jelly?
Guy 1: No for real she does.
Guy 2: AIN'T even Smuckers.
You ain't Smuckers by Brausten November 1, 2012
Related Words

You Ain't Got No Soap. 

Definition: 'you're full of it', 'whatever' or 'you're not that cool'. Soap is a valuable item in jail. Having a good bar of soap makes you a bit cooler, better/luckier than the rest of the inmates.
"I don't know why it's taking him so long to hang that new front door, damn, I would be finished already!"

His friend: " Oh you think you can hang doors now hu..? Psssh. Ha. You Ain't Got No Soap. "

Barnabas and Amy T

An extremely epic Christian rap group which originated in Seattle. Their iconic song "Head Down", a Calvary Chapel High School classic, has not only cured the depression of many lost souls (specifically those taking advanced classes in high school), but also the infamous Papa Wayne. They were totally 100% not obligated at all to perform at Calvary Chapel High School and other gigs. They are a married couple and have an epic gamer baby. As a high school kid Amy T did some ******* things which she would not approve of now (yes). Their music is 100% amazing and perhaps some of the greatest of all time.
Barnabas and Amy T by TheClorox December 17, 2018

ain't care 

When you give absolutely zero cares
Dude 1: "Dude, you just broke my South America topography map"

Chad: "ain't care"
ain't care by cadenski December 28, 2018

You Ain't Tough 

A Non-Tough guy who Actually isn't Strong, Brave
A Non-Tough guy who isn't a bully
And Ain't Stands for Are not/Is not/Am not
And you say it to a guy who trying to act That he's strong, brave, bully, Tough
And sometimes black people use it if they want beef with someone
Kid : Go away or i'll kill you stupid.
Teenager : u ain't tough! wanna beef?
White Kid : Hi Nigga
Black Guy : What did u just say???
White Kid : I said nigga you want a beef?
Black Guy : You ain't tough, wassup, *Punchs the white kid*
- What a savage kid tho.
Someone1 : Come on tough guy
Someone2 : YOU AIN'T TOUGH!
You Ain't Tough by Tanner Ethan January 24, 2019
I amn’t Gay
Amn’t by The_Paulsac April 10, 2019