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-when someone claims they did everything first.
-when someone says you are always copying them.
-shortened version of Johnny Joy killer or Joy Joy killer. (Meaning they ruin all the fun)
Bobby is pulling a JoJo. He thinks he invented air!

Stop being a Jojo, you didn't do it first.
A JoJo by ProperPeri June 12, 2022

A Jojo reference 

A reference to Hirohiko Araki's long running manga (and anime) series Jojo's Bizarre Adventure. Usually pertaining to things simular to or referencing Jojo's Bizarre Adventure themes such as floating or exploding Kanji, posing that covers the face and aesthetic of both the show and manga.
Hey Bob was posing at the business meeting today with his hands and hips. Was that his arthritis or a jojo reference?

A Jojo Reference 

Basically anything that comes out of a persons mouth
Person: *breathes*
WeeB: OHMYGOSH IS THAT A JOJO REFERENCE?

A Jojo Reference 

A Jojo reference is a reference to something in the Jojo's Bizarre Adventure Manga. Sometimes, people make Jojo references on accident, or on purpose. Like someone saying "I refuse." with the Rohan face, Or making a donut Joke.
Non-weeb: -Accidently does a Jojo reference without knowing-
Jackson: Is that a Jojo reference?
Non-weeb: Who the fuq is Jojo.
A Jojo Reference by Kakyoin Noriaki September 24, 2020

is that a jojo reference 

This phrase is usually said when a person or group makes a comment or question that refers to a element from jojo's bizarre adventure.
Guy 1: "Ay dude i ate a slice of bread"

Guy 2:"IS THAT A JOJO REFERENCE"