A reference to Hirohiko Araki's long running manga (and anime) series Jojo's Bizarre Adventure. Usually pertaining to things simular to or referencing Jojo's Bizarre Adventure themes such as floating or exploding Kanji, posing that covers the face and aesthetic of both the show and manga.
Hey Bob was posing at the business meeting today with his hands and hips. Was that his arthritis or a jojo reference?
by HentaiExpert August 30, 2017
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Increasingly used on the internet, the phrase "Is that a JoJo reference?!" refers to the anime JoJo's Bizzare Adventure. Since the anime is used in a lot of memes, when people spot something related to JoJo in a meme they tend to ask if it is a JoJo reference.
MEME: -Is a "to be continued meme", or says the word "NANI?!"-
Meme connoisseur: "Is that a JoJo reference?!!"
by supersonicsammyfan fan September 13, 2019
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This phrase is usually said when a person or group makes a comment or question that refers to a element from jojo's bizarre adventure.
Guy 1: "Ay dude i ate a slice of bread"

Guy 2:"IS THAT A JOJO REFERENCE"
by God of the defs June 21, 2020
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A Jojo reference is a reference to something in the Jojo's Bizarre Adventure Manga. Sometimes, people make Jojo references on accident, or on purpose. Like someone saying "I refuse." with the Rohan face, Or making a donut Joke.
Non-weeb: -Accidently does a Jojo reference without knowing-
Jackson: Is that a Jojo reference?
Non-weeb: Who the fuq is Jojo.
by Kakyoin Noriaki September 24, 2020
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A jojo reference is basically just everything that happent in jojo or just the sligtest resemblense to jojo
Gary: Hey! are you watching jojo?!
Harry: Yes why?
Gary: I HATE JOJO!
Harry: Sorry to tell you but thats a jojo reference! HA!
by Cheese.Man August 24, 2020
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