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1. A small, sweet yellow cake with a soft mixture like cream in the middle.

2. Informal, derogatory A homosexual or an effeminate man.
For meaning #2:

From "Friends":

Mrs. Geller: It seems your daughter and Richard are something of an item.
Mr. Geller: That's impossible. He's got a twinkie in the city.

Monica: Dad, I'm the twinkie.

Mr. Geller: You're the twinkie?

Richard: She's not a twinkie!
twinkie by Williamhhw October 8, 2015
An action where a male ejaculates into a female's nose. He then squeezes the sperm out of her nose by rubbing two fingers from the bridge of her nose to the tip. The sperm ends up in the female's mouth.
"Shannon and I were fooling around last night and she let me give her the twinkie."
Dre: What you get on your test?

Jerms: A twinkie.
Twinkie by iPodJunkee January 16, 2009
The cutest most adorable yorkie in the world. Very cute doggy. Black and brown Yorkie. Full of love and happiness.
OMG! Twinkie is just so darn cute and adorable! look at him wag his itty bitty tail!
Twinkie by fluffy ears February 22, 2010
A cute kangeroo formerly owned by Steph and Cliff, unfortunately Steph slaughtered it after it grew too big.
"Why Steph, WHY??! i miss Twinkie
Twinkie by cloudnine_vegas January 13, 2008
the TWINKIE was taking his blues and yellows to show his whore he was still 17

the TWINKIE was calling his whore day and night promissing a lot of money if she spent the night and wore a strap on to rock his world.

the TWINKIE had to get on all fours and get spanked because the thrill was gone otherwise.
twinkie by mumfucker June 15, 2008