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A term used in gaming, most likely stemming from MMORPG lingo, where shortening terms becomes essential for text communication. A debuff can be used in two similar respects. It replaces the drawn-out phrase "negative status ailment", where an attack or spell causes some ill effect on it's target, i.e. poisoning, silence, bleed. Debuff can also be used referring to an attack or spell that removes positive status effects. Many traditional RPGs have adopted this term as well.
The noob stood no chance against the terrifying onslaught of poison and slow debuffs, and was soon witnessed yelling "wee wee wee," all the way home.
debuff by Opus Atrum July 23, 2009
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1. Negative effect(s) placed on an avatar in a video-game world. (Usually used in MMO's).

1. To remove a 'buff' (MMORPG term used to describe positive effects on your avatar). This is a much less used application of the word 'debuff', the word 'dispel' is used more commonly instead.
God I fucking hate the Mortal Strike debuff, it makes healers almost worthless in Arenas!
debuff by Sharfeer March 12, 2008

Dobbie Debuff 

At least I don't have the dobbie debuff
Dobbie Debuff by Lost Ark Retards November 24, 2022

paychee debuff 

Paychee debuff is when a person or psyche throws an overwatch game, doesn't offer healing and becomes a DPS Moira.
"BRO OUR MOIRA ISN'T HEALING THIS IS SUCH A PAYCHEE DEBUFF"
paychee debuff by psycheeeey November 6, 2022

Dude Debuff

A debuff received by men in any gatcha waifu collector game where they are treated poorly by the devs and/or player base on accounting of being dudes. This usually harms 9 out of 10 husbandos.
that new 5 star's suffering from the dude debuff. everyone hates them for just being a guy.
Dude Debuff by Fox39 April 2, 2026
A mythical beneficial statistic awarded to severely over priced imaginary gear in a mobile game. A debuffer is a term used to bait unsuspecting gamers into spending thousands of dollars for the false hope that said debuffer, will debuff the gamers enemies buff.
Have no fear, im being rallied but I have my handy debuffer. Its gonna debuff their debuff against my double down dirty debuffer, which will negate the original buff.
Debuffer by MityMoose June 6, 2015

Dewuffing 

The act of simultaneously docking, fluffing, and webbing. In all practicality, quite hard to accomplish, due to the nature of the various tasks. Requires tact and skill, earning those that succeed at the act the lofty title of "dewuffers".
Bro, last night got so wild. I thought I was going to bang some fine chicks, but I ended up dewuffing with some creepy old french guy named Gaston. Clearly, I was homoblivious.
Dewuffing by Dock Master Rob February 17, 2009