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Increasingly used on the internet, the phrase "Is that a JoJo reference?!" refers to the anime JoJo's Bizzare Adventure. Since the anime is used in a lot of memes, when people spot something related to JoJo in a meme they tend to ask if it is a JoJo reference.
MEME: -Is a "to be continued meme", or says the word "NANI?!"-
Meme connoisseur: "Is that a JoJo reference?!!"
via giphy
by supersonicsammyfan fan September 13, 2019
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