It means that the target is in a bad mood or in unnecessary distress, and you are implying this with the notion of them having a stick in their anal cavity, which, of course, is very painful and would definately cause a rather pissy mood. It's a way of telling them to STFU. However, this phrase is not recommended around people who constantly make Yo Mama jokes. A similar thing to say would be telling them this with the Sandy Vagina phrase.
Guy 1: Dude, I have so much damn homework I'm gonna tear someone's freakin eyes out! Aaarrgh! *throws nearby freshman out a window*
Guy 2: Dude pull the stick out of your ass!
Guy 2: Dude pull the stick out of your ass!
by FunnyLolMan June 29, 2009
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Pull the stick out of your ass • pull out the stick • pull out the blicky and do a sticky • Mickey mouse fucks Minnie mouse in the asshole and loses his boner as he enters her urinary tract and gets stuck and has to pull out but his dick falls off and now he's gay • pull the trigger • pull the pin • pull the goalie • pull the hippo out of the mud • pull the plug • pull the devil trigger