"Ate my balls" or "I ate my balls" is an expression in the standup comedy community, whereby a comedian goes on stage and attempts to make an audience laugh, and fails miserably, also known as "bombing."
This can be due to either:
The comedian performing the material poorly.
-Or-
An obnoxious, aggressive, or just generally shitty crowd, or rather, that the crowd and the comedian weren't meshing, weren't on the same page comedically.
The expression is used in both cases. The expression seems to be popular with, and may have originated with comedians from the East Coast, particularly Boston; Bill Burr and Jim Norton are examples of comics who frequently use the expression.
This can be due to either:
The comedian performing the material poorly.
-Or-
An obnoxious, aggressive, or just generally shitty crowd, or rather, that the crowd and the comedian weren't meshing, weren't on the same page comedically.
The expression is used in both cases. The expression seems to be popular with, and may have originated with comedians from the East Coast, particularly Boston; Bill Burr and Jim Norton are examples of comics who frequently use the expression.
"Man this last open mic was brutal, I got up there and ate my balls in front of 20 other angry comics who looked like they were paid to be there."
by The Alaskan Assassin March 8, 2014
Get the Ate my ballsmug. by Kimmi998 June 3, 2010
Get the my balls are on firemug. by Richie January 27, 2004
Get the hummer my ballsmug. 
by Alistair_123 October 6, 2005
Get the sweating my ballsmug. by Muffdiver25 November 6, 2013
Get the Pulling my ballsmug. The act of farting on your balls to feel the pressurized air coming from your rectum and then shoot your balls with a butt burp
by Ordinary description January 27, 2023
Get the Freshing my ballsmug.