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1.
Whenever you deliver a witty remark to someone, and completely burn them, you say "Your move, sir/ma'am"
Some dude: Your mama is so stupid, when she threw a rock at the ground, she missed! Your move, dude.

Some OTHER dude: CHECKMATE sir!
by Not Zane August 03, 2004
 
2.
(interjection)
1. it is your turn to play as in chess, checkers or other games
2. it is your right to initiate the first attempt to draw a holstered gun as in a pistol duel (gun-slinging term)
The gunslinger told his opponent, "your move!"
by Jon64Bailey August 01, 2014