G- Get
N- Nob
O- Out

Thus literally meaning get your nob out
Usually used as an insult or as an obvious sex phrase
Boy: You're a loner and you've got no friends (Insulting)
Boy2:GNO

Or

Boy: Wanna have sex?
Girl: GNO!
by The Pro One March 11, 2009
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Bro ghoulnut and nigroid (rip 4k) are in GNO and make fire OC
by SpicyBoi2Dank4U September 30, 2018
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Gno (or gnoe, plural: gnoes) is a bastardized version of "No," applied in a manner to make it seem silly. The original objective was to parody people who were starting threads on message boards with topics like "OH NO!!!!!!1111"

It was first employed afterwards in limited amounts on chat sites, by people mocking other people that cannot spell - or at least those who choose not to.

Only recently has it really started to resurface on the web, and in particular, IRC. Jaimas, a regular IRC Chatter has repeatedly used it on his channel, and several others have taken a liking to the saying.
"Oh teh gnoes!"

"Oh gno!"
by Jaimas August 25, 2004
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When Saying "I Know." Just isn't enough.
Marissa: "There's Only 30 Days of school left!"

Holly: "I GNO I GNO"
by HollyxBoxBolly May 10, 2010
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