short for "most definitely" (contrary to popular belief it is NOT mo def, and whoever said that is fucking retarded), used because saying most definitely sounds gay and mo defs sounds cool
Kid 1: dude that girl is so hot
Kid 2: most definitely Kid 1: wtf
Kid 2: Uhh I mean mo defs
Kid 1: true dat Kid 3: mo def
Kids 1 and 2: ...
Kid 1: shut up dumbass
It is said of the situation where a person has the bad luck to make contact with his testicles against an undefined surface or object, intentioned or not.
Given the nature of the word, it is more appropriate to design cases where the interaction is made with a moving object, for example, a ball.
Although it is extremely painful for the victim, it tends to be considerably funny to people who witness it.
Today in the baseball game the pitcher took a nutshot; the baseball hit him in the nuts.
Man, I just watched the funniest nutshot video ever.