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In video gaming, the act of killing one's enemy using a fatal attack to their testicles.
Joe: DUDE! You just shot that terrorist in the balls!
Jamie: Chalk up another testikill!
by j loh August 24, 2007
To kill someone from testicle related injury. Often done via large calibre firearms.
That's it, I am going to testikill you right here, with my browning.
by Meok August 28, 2007