jimmies – feelings / thoughts / ego
rustled – hurt / disturbed
unrustled – peaceful / calm

As per Know Your Meme, the expression originated from 4chan in late 2010, and is used by trolls to "convey feelings of discontent or discomfort in response to someone else’s post in imageboards and discussion forums".

The expression is an alternate form of "u mad bro?".
 
Victim: What the heck is your problem!?
Troll: Are your jimmies rustled?
Victim: ...What is this I don't even
Troll: *trollface*
by AgentRev June 17, 2012
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Another name for the game commonly known as "Hacky Sack".
"Come on mate! Get out the jimmy and we can start rustling!"

"Nice Jimmy Rustling there man!"
by HacktheSack May 28, 2012
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Verb: To erk someone. To be all up in their biz. To cause frustration and anxiety. Basically to piss someone off in a profound way. Or, to bother someone's friends named jimmy
You: yo mama...
Me: I can't stand it when you rustle my jimmies like that

~or~
You: hey jimmy and jimmy. You suck
Me: Dude stop rustling my jimmies and apologize
by RustledJimmy January 14, 2016
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To bother or upset someone over a fairly trivial matter.
"have you been rustling someone's jimmies today?"

" yes, my friend got upset that I beat him yet again in monopoly"
by Soup dog March 15, 2015
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When something rustles your jimmies, it annoyed you or made you slightly angry.
Person 1: I can't believe Chipotle ran out of black beans!
Person 2: I know! That really rustles my jimmies.
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as others have stated: something has angered, upset, annoyed, aroused you
this is more of a side note on origin - I suspect the phrase was a spontaneous misquote of a line from the Weezer song "Say It Ain't So" ("...flip on the telly/ wrestle with Jimmy...")
man, that pic was disgusting.
yeah, that one rustled my jimmies
by kingprout1 July 31, 2015
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