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1.
The female version of jack off. To masturbate. Unassisted auto-erotic stimulation
Her boyfriend was out of town, so she got in the hot tub to Jane-off.
by TristanShout March 24, 2011
 
2.
When you masterbate while your penis is in pain like an elderly man.
I had a Jane Off last night and it hurt.
by jgsteroid50 January 14, 2011