Term used in Academic Team (don't laugh) where one team buzzes in early on a question and gets it wrong so the question is to be read in it's entirety to the other team. No one player on this team should buzz in early while the question is being read. Doing so will result in "Prime Directive," and angry stares from your teamates because you were supposed to wait until they knew what it was (jealous little shits).
I commit Prime Directive all the time because I am a power hungry Indian male and thats that's what Power Hungry Indian males do. It doesn't really matter because I always get the question right so get off my case, John!
John: Man, Kush! That's your third Prime Directive this tournament, you gotta learn to wait!
Kush: Shut the fuck up bitch, that's three more toss ups than you've scored.