twerk off Share definition Flag A battle between two twerkers in which they shake there ass vigorously till ones ass fall off deeming the other the winner. Damn bro did you see that chicks ass in the twerk off.. She totally knows how to shake it twerk off by Dirtboyz September 25, 2013 0 0 Get merch Get the twerk off mug. Buy Now — $32.95 See more merch