rootinest could be the way the word route sounded from cowhands,and tootinest could be the farts from all the beans they ate on the route or trail.Or and other wise you might even say rootinest means rowdiest instead of roughest.
If you ever saw Blazing Saddles,there would be an example of tooting around the camp fire.That was one rootinest tootinest time at the campfire.So I take exception to the meaning roughest and toughest.It don't make sense.It was an after dinner time thank you to the cook.
by alfred sinclair October 14, 2011
A yee haw, honkey tonk, redneck form of "roughest, toughest." Usually spoken with a mock Southern accent.
by Dr. Venkman December 10, 2005