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1.
–noun

1) -to overcome danger, adversity, difficulty with inner strength derived from ones testes.

2) Testosterone fueled resolve or action.
Guy 1: Man, I don't know if I could've set my girlfriend's house on fire after she dumped me.

Guy 2: That took serious testitude.
by Roger Brunswick November 27, 2008
 
2.
an evolved form of the phrase "intestinal fortitude"

inTESTInal fortiTUDE
that warrior has courage in the face of difficulty
that warrior has intestinal fortitude
that warrior has testitude
by big mac twista September 27, 2009