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You would use the term when you think it would be in the be in the best interest of the party/s or person/s confronting you to leave and/or leave you alone. It should be stated with feeling.
You best step-off, mother fucker!
by W. Steward April 20, 2004
back away from a confrontation.
by Mr. Terrence L. Trezvant October 05, 2003
Church for gangsters.
"You better go to Step Off and pray"
by asian_gangsters November 03, 2008