A replacement for the classic but tired adage of "that's what she said". It was developed from Demetri Martin's comedy, which states that adding "...ladies" at the end of anything will make it creepier. It can be added by the person talking or anyone who happens to be listening and catches a double entendre ready to be exploited.
Ray: Jeeze, I'm really tired from last night...ladies.
Harrison: I didn't think it was going to be this hard!