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Its an alternate form of saying the word "Taint."
She tickled the area near my undercarriage that twasnt my balls or ass.
Twasn't by Steve December 13, 2003

'twasn't 

An alternative of "it wasn't"
Someone: I think cars 3 was a good movie!

Logan: 'twasn't
'twasn't by I am not a female June 26, 2023

Pseudofightantigigantophobialisn'taren'twasn'tcouldn'tshouldn'twon'tdon'tdidn't 

A state of performative resistance in which one outwardly postures as a challenger of great forces—be they literal giants, metaphorical fears, or societal systems—while internally constrained by a complex web of doubts, prohibitions, and hypothetical counterfactuals. The subject inhabits a mental space where action is forever deferred by a litany of imagined limitations: one could fight, but one can’t; one might oppose, but one shouldn’t; one dreams of standing tall, but wouldn’t dare; and ultimately, one won’t—not now, not ever.
"NO, EVERY DAY I DO ALL THE WORK AND YOU JUST Pseudofightantigigantophobialisn'taren'twasn'tcouldn'tshouldn'twon'tdon'tdidn't"

T'wasn't 

The Old English new age combination of the words: It, Was, and Not.
"What night did that burglar break into the house?"

"T'wasn't the night before Christmas."

"You're a douche."
T'wasn't by Benjiforce1 October 1, 2010

'Twas ain't

A phrase that combines the contractions 'twas (it was) and ain't (is not), closely meaning "It was not" or "It is not".

In a casual conversation appropriate use would be in the context of disagreement or disapproval.

On January 6, 2008, on a surprisingly warm Sunday evening (A stark contrast from the frigid cold one had to endure the previous days) under the influence of a particular, earthy substance, two Chicago residents named Jason and Carla spoke of fancied and eloquent discourse in the comfort of Carla's bedroom.

As their conversation ran it's natural course, Carla verbally penetrated Jason's psyche, and in doing so planted the seed to would blossom into divine inspiration. Then from the mind to the voice came the words "'Twas ain't!, birthed from Jason's mouth.

Laughter ensued.
Carla: This particular term sounds concurrently Shakespearean and CRUNK!

Jason: 'Twas AIN'T! More specifically, surely our lovechild that is this newfound phrase contains within itself allusions of both Shakespeare and contemporary, Black-urban vernacular.

whomst'd've'lu'yaint'nt'ed'ies's'y'es'nt't're'ing'able'tic'ive'al'nt'ne'm'll'ble'al'ny'less'w'ck'k'ly'py'nd'idy'ety'st'ged'ful'ish'ng'mt'ous'er'tis'twas'all'terent'djfignick'fed'sie'yenas'ighohocasaldingtariunistic'iuds'mentarsariot 

The superior version of saying Whomst'd've.
Created in order to show superiority over peers or younglings.
GUY 1: Yo, do you have any idea whomst'd've'lu'yaint'nt'ed'ies's'y'es'nt't're'ing'able'tic'ive'al'nt'ne'm'll'ble'al'ny'less'w'ck'k'ly'py'nd'idy'ety'st'ged'ful'ish'ng'mt'ous'er'tis'twas'all'terent'djfignick'fed'sie'yenas'ighohocasaldingtariunistic'iuds'mentarsariot took my notebook?

GUY 2: ....What?