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It means that the target is in a bad mood or in unnecessary distress, and you are implying this with the notion of them having a stick in their anal cavity, which, of course, is very painful and would definately cause a rather pissy mood. It's a way of telling them to STFU. However, this phrase is not recommended around people who constantly make Yo Mama jokes. A similar thing to say would be telling them this with the Sandy Vagina phrase.
Guy 1: Dude, I have so much damn homework I'm gonna tear someone's freakin eyes out! Aaarrgh! *throws nearby freshman out a window*
Guy 2: Dude pull the stick out of your ass!
by FunnyLolMan June 29, 2009

Words related to Pull the stick out of your ass

sandy vagina yo mama ass pull stfu stick