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1.
A Catholic service fraternity for Catholic men aged 18 and up. Started by the Venerable Father Micheal J McGivney in New Haven Connecticut, the Knights of Columbus is a service organization which Catholic men participate to support the Roman Catholic Church and people in need of service. Often called "the strong Right arm of the Church", knights work in humble service for Christ's Church on earth.
See those guys at the front of the march for life? They're Knights of Columbus.
by Cup0Joe March 11, 2013
 
2.
Expression used when one is in a great deal of pain.
"Knights of Columbus that hurt!!"
"Knights of Columbus, I've been shot!"
by Jonny Cheecho January 27, 2008